polyclonal rabbit anti human b cell lymphoma 2  (Boster Bio)

Journal: Oncology Letters

Article Title: ZBTB20 suppresses tumor growth in glioblastoma through activating the TET1/FAS/caspase‑3 pathway

doi: 10.3892/ol.2024.14491

Figure Lengend Snippet: ZBTB20 promotes apoptosis in glioblastoma cells. (A) Western blotting was used to detect BCL2, BAX, CASP3 and cleaved CASP3 expression in ZBTB20-overexpressing U251 cells, (B) the levels of which were semi-quantified and are presented as histograms. The independent experiment was repeated three times. Data are presented as the mean ± standard deviation. *P<0.05. BCL2, B-cell lymphoma-2; BAX, BCL2 associated X; CASP3, caspase-3; NC, negative control; ZBTB20, zinc finger and BTB domain containing 20.

Article Snippet: Next, the membranes were separately incubated overnight at 4°C with the following antibodies: Polyclonal rabbit anti-human ZBTB20 (dilution, 1:2,000; cat. no. ab127702; Abcam), polyclonal rabbit anti-TET1 (1:1,000; cat. no. A21766; ABclonal Biotech Co., Ltd.), polyclonal rabbit anti-human FAS (1:1,000; cat. no. A2639; ABclonal Biotech Co., Ltd.), polyclonal rabbit anti-human B-cell lymphoma-2 (BCL2; 1:2,000; cat. no. BA0412; Wuhan Boster Biological Technology, Ltd.), polyclonal rabbit anti-human BCL2 associated X (BAX; 1:2,000, cat. no. BA0315-2; Wuhan Boster Biological Technology, Ltd.), monoclonal rabbit anti-human CASP3 (1:1,000, cat. no. AF1213; Beyotime Institute of Biotechnology), monoclonal rabbit anti-human cleaved CASP3 (1:1,000; cat. no. AF1150; Beyotime Institute of Biotechnology), monoclonal rabbit anti-human phosphorylated-extracellular signal regulated kinase (p-ERK; 1:1,000; cat. no. AP0485; ABclonal Biotech Co., Ltd.), polyclonal rabbit anti-human ERK (1:1,000; cat. no. A16686; ABclonal Biotech Co., Ltd.), monoclonal rabbit anti-human p-cyclic AMP-responsive-element-binding protein (CREB; 1:500; cat. no. ab32096; Abcam), monoclonal mouse anti-human CREB (1:500; cat. no. ab178322; Abcam) and monoclonal rabbit anti-human β-actin (1:10,000; cat. no. AC026; ABclonal Biotech Co., Ltd.).

Techniques: Western Blot, Expressing, Standard Deviation, Negative Control

Journal: Marine Drugs

Article Title: In Vitro Prebiotic and Anti-Colon Cancer Activities of Agar-Derived Sugars from Red Seaweeds

doi: 10.3390/md19040213

Figure Lengend Snippet: Induction of apoptosis in HCT-116 human colon cancer cells by AHG. ( A ) 4′,6-Diamidino-2-phenylindole (DAPI) staining of HCT-116 cells treated with AHG. ( B – E ) Western blot results showing the effects of AHG on expression of apoptosis-related proteins in cancer cells: procaspase-3 ( B ), caspase-3 ( B ), procaspase-9 ( B ), caspase-9 ( B ), poly(ADP-ribose) polymerase (PARP) ( B ), cleaved PARP ( B ), Bcl-2 ( C ), Bcl-xL ( D ), Bax ( D ), and p53 ( E ). β-actin was used as a control for all Western blot experiments. The relative intensities were quantified using an Image J program and normalized by the intensity of β-actin. Data are mean ± SD relative to the untreated group. *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. untreated control.

Article Snippet: Next, the following antibodies purchased from Cell Signaling Technology (Danvers, MA, USA) were used to determine expression levels of the indicated proteins: rabbit polyclonal anti-human caspase-3, rabbit polyclonal anti-human caspase-9, rabbit polyclonal anti-human PARP, rabbit polyclonal anti-human Bcl-2, rabbit polyclonal anti-human Bcl-xL, rabbit polyclonal anti-human Bax, rabbit monoclonal anti-human p53, and mouse monoclonal anti-β-actin.

Techniques: Staining, Western Blot, Expressing